Syringe Filters Overview

Luknova supplies a variety of high quality syringe filters (with female luer lock inlet and male slip outlet) for sample preparation and solvent filtration to remove particles from a sample prior to chromatography, filtration of gases, and for the removal of bacteria from a aqueous and non-aqueous sample, including Nylon, PTFE (polytetrafluoroethylene), PVDF (polyvinyldifluoride), PES (Polyethersulfone), and MCE (mixed cellulose acetate and cellulose nitrate).




 Click following tab to display product description and part numbers.


Order Information for Syringe Filters

Membrane Type Diameter 13 mm Diameter 25 mm Diameter
Pore Size 0.22µm 0.45µm 0.22µm 0.45µm
Nylon 100/PK L10013022A L10013045A L10025022A L10025045A
500/PK L10013022B L10013045B L10025022B L10025045B
PTFE (polytetrafluoroethylene) 100/PK L11013022A L11013045A L11025022A L11025045A
500/PK L11013022B L11013045B L11025022B L11025045B
PVDF (polyvinyldifluoride) 100/PK L12013022A L12013045A L12025022A L12025045A
500/PK L12013022B L12013045B L12025022B L12025045B
PES (Polyethersulfone)  100/PK L13013022A L13013045A L13025022A L13025045A
500/PK L13013022B L13013045B L13025022B L13025045B
MCE (mixed cellulose acetate and cellulose nitrate)  100/PK L14013022A L14013045A L14025022A L14025045A
500/PK L14013022B L14013045B L14025022B L14025045B


Order Information for Membranes

Membrane Type    Diameter 13 mm Diameter 25 mm Diameter 47 mm Diameter
Pore Size 0.22µm 0.45µm 0.22µm 0.45µm 0.22µm 0.45µm
Nylon 100/PK L15013022A L15013045A L15025022A L15025045A L15047022A L15047045A
PTFE 100/PK L16013022A L16013045A L16025022A L16025045A L16047022A L16047045A
PVDF 100/PK L17013022A L17013045A L17025022A L17025045A L17047022A L17047045A
PES 100/PK L18013022A L18013045A L18025022A L18025045A L18047022A L18047045A
MCE 100/PK L19013022A L19013045A L19025022A L19025045A L19047022A L19047045A


Syringe Filters Material Characteristics 

Membrane Name Nylon PTFE PVDF PES MCE
Membrane Type Hydrophilic Hydrophobic Hydrophilic Hydrophilic Hydrophilic
Effective Filtration Area 0.63 cm2 (for 13mm) and 3.5 cm2 ( for 25mm)
Hold-up Volume <15µL (for 13mm) and <75µL (for 25mm)
Housing Polypropylene
Inlet/Outlet Connections Female luer lock inlet, Male slip luer outlet
Maximum Operating Temperature Maximum 100 °C (212 °F) at 2.1 bar (210 kPa, 30 psi)
Sterilization Provided non-sterile. If needed, autoclave at 121°C (250°F) at 1.0 bar (100 kPa, 15 psi) for a maximum of 15 min.


Filter and Membrane Characteristics  

Filter Sizes (diameter)  13mm and 25 mm
Filter Pore Sizes 0.22 µm and 0.45 µm
Filter Membrane Sizes (diameter)  13mm, 25mm, and 47mm

Syringe Filter Selection Guide

Step 1 Select Filter Diameter

Sample volume amount ≤ 10 mL 10-100mL
Syringe filter diameter 13mm 25mm


Step 2 Select Pore Size of the Filter Membrane

Sample physical nature and analytical methods Filter membrane pore size
Viscous materials or samples that contain high concentrations of solid particulate matter and mobile phase filtration 0.45 µm
HPLC samples to be analyzed with column packed with > 3µm materials and GC, SFC, CE, GPC, and Flash samples 0.45 µm
HPLC samples to be analyzed with column packed with < 3µm materials and GC, SFC, CE, GPC, and Flash samples 0.22 µm
General samples that contain fine particulate matter prior to GC, SFC, CE, GPC, and Flash samples, and sterile-filtration 0.22 µm
Gas, liquid sample and solvent prior to GC, LC/MS and UHPLC, and other methods sensitive to particulate 0.22 µm


 Step 3 Select Filter Membrane According to the Sample Characteristics and Filtering Objective pore size

Membrane Type Recommended Application
Nylon Hydrophilic and commonly used for aqueous or mixed organic sample prep and HPLC, GC or dissolution sample analysis, such as bases, most HPLC solvents, alcohols, aromatic hydrocarbons, and THF.  Not for strong acids, strong bases and high protein recovery.  Excellent flow rates with most sample matrices and extremely low in extractables.
PTFE (Teflon®) Hydrophobic and perfect for organic solvent-based, acidic or basic samples and all solvents, such as aggressive solvents, strong acids and bases, alcohols, and aromatics.  Chemically resistant to all solvents and has an excellent thermal stability to high temperature fluids.  It can be used with aqueous samples after pre-wetting with small amount of alcohol and then flushing with water.
PVDF Hydrophilic and excellent for HPLC and GC sample prep/clean up and protein-based samples due to broad chemical compatibility, the nature of a low protein binder, and low UV adsorbing extractables.  It can be used for alcohols, weak acids, proteins, peptides and other biomolecules for high protein recovery .
PES Hydrophilic and excellent for tissue culture, media, and buffers due to very low protein and nucleic acid binding and excellent flow rates. The PES membrane shows better chemical resistance than cellulose acetate.  It is widely used in clinical/toxicology, ion chromatography, ICP-MS, AAS, and capillary electrophoresis for strong bases, alcohols, proteins, peptides.
MCE Hydrophilic and ideal for aqueous samples filtration that need higher flow rates and larger volume, including clarification or sterilization of aqueous solutions, particulate analysis and removal, air monitoring, microbial analysis, cytology, HPLC samples prep/clean up, virus concentration, biological assays, food microbiology (enumeration of E. coli in foods), bacteriological studies.


Syringe Filter Chemical Compatibility 


Acids Halogenated Hydrocarbons
Acetic, Glacial + v v v × Methylene Chloride + v v × ×
Acetic, 25% v v v v v Chloroform v v v × ×
Hydrochloric, Concentrated × v v v × Trichloroethylene v v v × v
Hydrochloric, 25% × v v v × Monochlorobenzene v v v + v
Sulphuric, Concentrated × v × × × Freon® v v v + v
Sulphuric, 25% × v v v × Carbon Tetrachloride v v v × +
Nitric, Concentrated × v v × × Hydrocarbons
Nitric, 25% × v v v × Hexane, Xylene v v v × v
Phosphoric, 25% × v ND ND v Toluene, Benzene v v v × v
Formic, 25% × v ND ND + Kerosene, Gasoline v v v + v
Trichloroacetic, 25% × v ND ND v Tetralin, Decalin ND v v ND v
Alcohols Ketones
Methanol, 98% v v v v v Acetone v v × × ×
Ethanol, 98% v v v v v Cyclohexanone v v × × ×
Ethanol, 70% + v v v v Methyl Ethyl Ketone v v + × +
Isopropanol v v v v v Isopropylacetone - v v × × v
n-Propanol v v v v v Methyl Isobutyl Ketone ND v + × ND
Amyl Alcohol, Butanol v v v v v Organic Oxides
Benzyl Alcohol v v v ND + Ethyl Ether v v v v v
Ethylene Glycol v v v v v Dioxane v v + × +
Propylene Glycol v v v v + Tretrahydrofuran v v + × +
Glycerol v v v v v Triethanolamine v v ND ND v
Alkalis Dimethylsulfoxide (DMSO) v v × × +
Ammonium Hydroxide, 25% v v + v v Isopropyl Ether ND v v v v
Sodium Hydroxide, 25% v v v v × Miscellaneous
Amines and Amides Phenol, Aqueous, 10% ND v + × ×
Dimethyl Formamide + v × × × Formaldehyde Solution, 30% v v v v v
Diethylacetamide v v ND ND × Hydrogen Peroxide, 30% v v ND ND v
Triethanolamine v v ND ND v Silicone Oil & Mineral Oil ND v v v v
Aniline ND v ND ND ×  
Pyridine v v × × ×
Acetronitrile v v v + ×
Esters Legend Description
Ethyl & Methyl Acetate v v v × × PTFE Polytetrafluoroethylene
Amyl & Butyl Acetate v v × × + PVDF Polyvinyldifluoride
Propyl Acetate v v × × + PES Polyethersulfone
Propylene Glycol Acetate ND v ND × × MCE Mixed cellulose acetate and cellulose nitrate
2-Ethoxyethyl Acetate ND v ND × + v Compatible
Methyl Cellosolve Acetate ND v ND × × + Limited compatibility (membrane may  swell and shrink), use with caution
Benzyl Benzoate v v ND × v
Isopropyl Myristate v v ND × v × Incompatible (not recommended)
Tricresyl Phosphate ND v ND × v ND No compatible data available

About Syringe Filters

A syringe filter is a single-use filter cartridge. It is typically attached to the end of a syringe for use. Luknova syringe filters have Luer lock fittings. The use of a needle is optional; where desired it may be fitted to the end of the syringe filter.


A syringe filter generally consists of a plastic housing with a membrane which serves as a filter. The fluid to be purified may be cleaned by drawing it up the syringe through the filter, or by forcing the unfiltered fluid out, through the filter.



  1. SDS of Syringe Filter Products.pdf


Special Request and Contact Us

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